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Multivalent Designed Proteins Neutralize SARS-CoV-2 Variants of Concern and Confer Protection against Infection in Mice

Although adoption of CFE systems is increasing, their use in most laboratories is far from routine

The note should also frame the result clearly

Samples each containing approximately 500 g of protein in SDC lysis buffer (100 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid [HEPES], 1% sodium deoxycholate [SDC], pH 8.1) were reduced and alkylated using 10 mM tris(2-carboxyethyl)phosphine (TCEP Sigma Aldrich) and 40 mM of chloroacetamide (CAA) at 95 C for 5 min

These findings are indicative of increased membrane repair capacity and are consistent with a decrease in hemolytic propensity in supercooled RBCs when stored in this additive as opposed to standard SAGM

The obtained black product was centrifuged, and then thoroughly washed with distilled water and absolute ethyl alcohol, respectively